FOXO4-DRI - CAS:2460055-10-9

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FOXO4-DRI

目录号 : KP1519 CAS No. : 2460055-10-9

Data Sheet 产品使用指南

FOXO4-DRI 是一种细胞渗透性肽拮抗剂，可阻断 FOXO4 和 p53 的相互作用。FOXO4-DRI 是一种抗衰老多肽，可诱导衰老细胞凋亡 (apoptosis)。

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

FOXO4-DRI is a cell-permeable peptide antagonist that blocks the interaction of FOXO4 and p53. FOXO4-DRI is a senolytic peptide that induces apoptosis of senescent cells.

体外研究

FOXO4-DRI (25 mM; 3 days) induces nuclear exclusion of active p53 and triggers apoptosis in senescent TM3 Leydig cells [1].
FOXO4-DRI (25 μM; 5 days) significantly reduced the senescence level of PDL9 cells [2].

Cell Viability Assay

Cell Line:

Senescent Leydig cells

Concentration:

25 mM

Incubation Time:

3 days

Result:

Reduced the viability of senescent as compared to normal TM3 Leydig cells.

Apoptosis Analysis

Cell Line:	Senescent Leydig cells
Concentration:	25 mM
Incubation Time:	3 days
Result:	The apoptosis rate increased from 10% to 27%.

Western Blot Analysis

Cell Line:	PDL9 cells
Concentration:	25 μM
Incubation Time:	5 days
Result:	Decreased the protein levels of representative senescent markers, including p16, p21, and p53.

RT-PCR

Cell Line:	PDL9 cells
Concentration:	25 μM
Incubation Time:	5 days
Result:	Enhanced SOX9 expression, and reduced MMP12 and MMP13 expression.

体内研究

FOXO4-DRI (5 mg/kg; i.p.; administered every other day for three doses) alleviates testosterone deficiency in naturally aging mice and improves the testicular microenvironment.

Animal Model:	Naturally aged male C57BL/6 mice (20-24 months old)
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection, every other day for three administrations
Result:	Increased serum testosterone levels. Increased levels of both 3β-HSD and CYP11A1. Decreased interstitial SA-β-gal activity and lowered levels of senescence-associated proteins p53, p21, and p16. Decreased the levels of IL-1β, IL-6 and TGF-β.

分子式

C₂₂₈H₃₈₈N₈₆O₆₄

分子量

5358.06

CAS号

2460055-10-9

中文名称

FOXO4-DRI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H2O : 100 mg/mL (18.66 mM; Need ultrasonic)

DMSO : 50 mg/mL (9.33 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.1866 mL	0.9332 mL	1.8663 mL
5 mM	0.0373 mL	0.1866 mL	0.3733 mL
10 mM	0.0187 mL	0.0933 mL	0.1866 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。