4-Vinylcyclohexene dioxide - CAS:106-87-6

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4-Vinylcyclohexene dioxide (Synonyms:Vinylcyclohexene diepoxide)

目录号 : KP18217 CAS No. : 106-87-6 纯度 : 98%

Data Sheet SDS 产品使用指南

4-Vinylcyclohexene dioxide (4-Vinylcyclohexene diepoxide) 是口服活性的 4-vinylcyclohexene 代谢物。4-Vinylcyclohexene dioxide 诱导凋亡 (Apoptosis)，增加细胞内 ROS，激活 PI3K/Akt/mTOR 通路。4-Vinylcyclohexene dioxide 选择性损伤卵巢小卵泡、抑制颗粒细胞功能及破坏雄性生殖系统。4-Vinylcyclohexene dioxide 可用于早发性卵巢功能不全、生殖毒性及相关 fertility 障碍的研究。

规格	价格	是否有货	数量
1g		In-stock
5g		In-stock
10g		In-stock
25g		In-stock

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生物活性

4-Vinylcyclohexene dioxide (4-Vinylcyclohexene diepoxide) is an orally active metabolite of 4-vinylcyclohexene. 4-Vinylcyclohexene dioxide induces Apoptosis, increases intracellular ROS, and activates the PI3K/Akt/mTOR pathway. 4-Vinylcyclohexene dioxide selectively damages small ovarian follicles, inhibits granulosa cell function, and disrupts the male reproductive system. 4-Vinylcyclohexene dioxide can be used in the study of premature ovarian insufficiency, reproductive toxicity, and related fertility disorders.

体外研究

4-Vinylcyclohexene dioxide (90 μM-3 mM; 24 h) induces apoptosis, increases intracellular reactive oxygen species, and inhibits proliferation in human ovarian granulosa cell line (SVOG).
4-Vinylcyclohexene dioxide (10 nM-10 mM; 6-24 h) promotes the activation of primordial follicles through transient activation of the PI3K/Akt/mTOR pathway.

Apoptosis Analysis

Cell Line:

Human ovarian granulosa cell line (SVOG)

Concentration:

0.5 mM, 1 mM

Incubation Time:

24 hours

Result:

Increased apoptosis with higher Annexin V-positive cells.
Induced a significant increase in Hoechst 33342 and PI positive cells in a dose-dependent manner.

体内研究

4-Vinylcyclohexene dioxide (80 mg/kg; intraperitoneal injection; Once daily, 1-15 days) accelerates follicular atresia by activating the caspase cascade in the preantral follicles of female F344 rats.
4-Vinylcyclohexene dioxide (80 mg/kg; intraperitoneal injection; 5-10 days) can promote superovulation in middle-aged C57BL/6J mice, increase serum E2 and FSH levels, and enhance follicular development.
4-Vinylcyclohexene dioxide (100-500 mg/kg; oral; Once daily for 28 days, it can disrupt the sperm characteristics in the testes and epididymis of male Wistar rats, reduce reproductive hormone levels, and induce oxidative stress and cell apoptosis.

Animal Model:	Female F344 rats (28-day-old)
Dosage:	80 mg/kg
Administration:	i.p., 1 day or 15 days
Result:	Increased cytosolic caspase-3 activity in small follicles (Day 1: 2.86; Day 15: 3.25, VCD/control). Increased procaspase-3 protein by 212% (Day 15). Increased caspase-8 activity (Day 15). Increased caspase-9 activity (Day 1).

分子式

C₈H₁₂O₂

分子量

140.18

CAS号

106-87-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 50 mg/mL (356.68 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	7.1337 mL	35.6684 mL	71.3369 mL
5 mM	1.4267 mL	7.1337 mL	14.2674 mL
10 mM	0.7134 mL	3.5668 mL	7.1337 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 15% Cremophor EL → 85% Saline

Solubility: 25 mg/mL (178.34 mM); Suspended solution; Need ultrasonic
2.

请依序添加每种溶剂： corn oil

Solubility: 25 mg/mL (178.34 mM); Clear solution; Need ultrasonic
3.

请依序添加每种溶剂：10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 5 mg/mL (35.67 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (35.67 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μL Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL。
4.

请依序添加每种溶剂：10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 5 mg/mL (35.67 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (35.67 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。

5.

请依序添加每种溶剂：10% DMSO → 90% corn oil

Solubility: ≥ 5 mg/mL (35.67 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (35.67 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。