Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C.
Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm.
Immunofluorescence.
| Cell Line: |
HeLa cells. |
| Concentration: |
1 μM. |
| Incubation Time: |
2 h. |
| Result: |
Mito-TRFS is localized predominantly in the mitochondria of live cells. |