TMRM Perchlorate - CAS:115532-50-8

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TMRM Perchlorate (Synonyms:T668)

目录号 : KM13543 CAS No. : 115532-50-8 纯度 : 98%

Data Sheet SDS 产品使用指南

TMRM Perchlorate 是一种细胞渗透性阳离子亲脂性红色荧光染料，可对线粒体膜电位进行特异性识别，从而附着在线粒体中，并产生明亮荧光，在一定浓度下，TMRM Perchlorate对细胞具有低毒性，因此普遍用于检测动物细胞、植物细胞以及微生物中的线粒体。 (激发波长 (Ex)=530 nm, 发射波长 (Em)=592 nm)。

规格	价格	是否有货
5mg		In-stock
10mg		In-stock
25mg		In-stock
50mg	询价	In-stock
100mg	询价	In-stock

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生物活性

TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, TMRM Perchlorate have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.(λ_ex=530 nm, λ_em=592 nm).

体外研究

TMRM Perchlorate is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of TMRM Perchlorate shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM Perchlorate (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%. Exposure of hippocampal cultures to low concentrations of TMRM Perchlorate (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM Perchlorate (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM Perchlorate (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (K_D = 500 nM).

分子式

C₂₅H₂₅ClN₂O₇

分子量

500.93

CAS号

115532-50-8

中文名称

四甲基罗丹明甲酯高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8˚C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 41.67 mg/mL (83.19 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9963 mL	9.9814 mL	19.9629 mL
5 mM	0.3993 mL	1.9963 mL	3.9926 mL
10 mM	0.1996 mL	0.9981 mL	1.9963 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

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