Macranthoidin B - CAS:136849-88-2

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Macranthoidin B (Synonyms:灰毡毛忍冬皂苷乙;Macranthoiside I)

目录号 : KM19806 CAS No. : 136849-88-2 纯度 : ≥98%

Data Sheet SDS 产品使用指南

灰毡毛忍冬皂苷乙(Macranthoidin B)是一活性天然皂苷。

规格	价格	是否有货
5mg		In-stock
10mg		In-stock
25mg		In-stock
50mg		In-stock
100mg	询价	In-stock
200mg	询价	In-stock

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生物活性

Macranthoidin B is a major bioactive saponin in rat plasma after oral administration of extraction of saponins from Flos Lonicerae.

体外研究

Macranthoidin B (0-500 μM; 24 h) can inhibit the migration and invasion of primary endometriosis stromal cells and HEC1-B endometrial cancer cells, as well as suppress epithelial mesenchymal transition and COX-2/PGE2 pathway.
Macranthoidin B (20-400 μM; 24-48 h) dose dependently reduces the survival rate of HCT-116 colorectal cancer cells, induces cell apoptosis, and promotes ROS generation.
Macranthoidin B (20-400 μM; 48 h) can dose dependently promote ROS generation in HCT-116 colorectal cancer cells.
Macranthoidin B (20-400 μM; 48 h) can dose dependently downregulate the mRNA expression of antioxidant enzymes MnSOD and GSH Px in HCT-116 colorectal cancer cells.
Macranthoidin B (100-400 μM; 24 h) can dose dependently inhibit Hepa1-6 cell proliferation and increase intracellular ROS levels.
Macranthoidin B (100-400 μM; 24 h) can dose dependently induce oxidative stress in Hepa1-6 cells, manifested by a significant increase in MDA levels and a significant decrease in GSH levels, SOD activity, and GSH Px activity at all tested concentrations.

Cell Viability Assay

Cell Line:

HCT-116 colorectal cancer cells

Concentration:

20, 50, 100, 200, 400 μM

Incubation Time:

48 hours

Result:

Caused a dose-dependent decrease in cell viability, with significant reductions observed at all tested concentrations relative to control.

Western Blot Analysis

Cell Line:	HCT-116 colorectal cancer cells
Concentration:	20, 50, 100, 200, 400 μM
Incubation Time:	72 hours
Result:	Altered caspase-3 expression, increasing levels of cleaved caspase-3 in a dose-dependent manner.

体内研究

Macranthoidin B (3-27 mg/kg; gavage; Daily; 28 days) can inhibit the growth of ectopic lesions in a rat model of endometriosis after autologous transplantation.
Macranthoidin B (16 mg/kg; intraperitoneal injection; Once a day; Lasting for 3 weeks, it can significantly inhibit the growth of nude mouse colorectal cancer xenografts by inducing tumor cell apoptosis.

Animal Model:	Sprague-Dawley (SD) (female, 6-7 weeks old, 180-220 g, autograft implantation of endometrial segments endometriosis model)
Dosage:	3 mg/kg; 9 mg/kg; 27 mg/kg
Administration:	p.o.; daily; 28 days
Result:	Reduced post-treatment ectopic endometrial volume compared to pretreatment. Resulted in transplants with less adhesion and fewer surface blood vessels (9 and 27 mg/kg doses). Improved pathological morphology of ectopic tissues, with fewer and smaller pseudo glands, reduced microvascularity, and decreased inflammatory cell infiltrations. Decreased serum estradiol (E2) levels and increased serum progesterone (PROG) levels compared to the untreated endometriosis group. Increased mRNA and protein expression of epithelial marker Cdh1/E-cadherin in ectopic tissues. Decreased mRNA expression of mesenchymal markers Cdh2, Vim, Twist, Slug, Snail, Zeb1, and Zeb2, as well as protein expression of mesenchymal markers N-cadherin and Vimentin in ectopic tissues. Reduced mRNA expression of Ptgs2 and Ptges, and decreased protein levels of COX-2 and PGE2 in ectopic tissues.

Animal Model:	Nude mice (4 weeks old; half male, half female; engrafted with human HCT-116 colorectal cancer cells)
Dosage:	16 mg/kg
Administration:	i.p.; once daily; 3 weeks
Result:	Reduced tumor volume significantly compared to vehicle control. Reduced tumor weight significantly compared to vehicle control. Increased TUNEL-positive cells per field significantly compared to vehicle control. Elevated levels of cleaved caspase-3 compared to vehicle control.

分子式

C₆₅H₁₀₆O₃₂

分子量

1399.52

CAS号

136849-88-2

中文名称

灰毡毛忍冬皂苷乙；灰毡毛忍冬皂苷 B

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (71.45 mM; Need ultrasonic)

H2O : 100 mg/mL (71.45 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.7145 mL	3.5727 mL	7.1453 mL
5 mM	0.1429 mL	0.7145 mL	1.4291 mL
10 mM	0.0715 mL	0.3573 mL	0.7145 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：PBS

Solubility: 100 mg/mL (71.45 mM); Clear solution; Need ultrasonic

2.

请依序添加每种溶剂： 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
3.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
4.

请依序添加每种溶剂： 10% DMSO → 90% corn oil

Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。