NHI 2 - CAS:1269802-97-2

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NHI 2 (Synonyms:NHI 2)

目录号 : KCM10892 CAS No. : 1269802-97-2 纯度 : ≥98%

Data Sheet 产品使用指南

NHI 2 is an inhibitor of lactate dehydrogenase A (LDHA; IC = 14.7 µM). It is selective for LDHA over LDHB (IC = 55.8 µM). It inhibits lactate production in HeLa cells when used at concentrations of 100 and 200 µM. NHI 2 is cytotoxic to HeLa cells (IC = 33.4 µM).

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1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 of 14.7 µM. NHI-2 shows selective for LDHA over LDHB (IC50 = 55.8 µM). NHI-2 is an efficient anti-glycolytic agent. NHI-2 enhances apoptosis, induces cell cycle arrest at S and G2 phases. NHI-2 has a broad spectrum anti-proliferative activity in cancer cells. NHI-2 affects extracellular acidification rate and ATP production. NHI-2 suppresses tumor growth in murine B78 melanoma tumor model.

体外研究

NHI-2 (50-200 μM, 8 h) inhibits lactate production in HeLa cells [1].
NHI-2 (12-40 μM, 72 h) inhibits the proliferation of B78 cells [2].
The EC50 of NHI-2 (0.1-100 μM, 48 h) on B78 cells was 32 μM [2].
The combination of NHI-2 (40 μM, 24 h) with 30 or 60 mM Oxamate (Oxa) (HY-W013032A) enhances apoptosis [2].
NHI-2 (40 μM, 24 h) induces cell cycle arrest at the S/G2 phase [2].
NHI-2 affects extracellular acidification rate (ECAR) (12-26 μM, 80 min) and ATP (18 μM, 24 h) production, and synergizes with Oxa [2].
As a competitive inhibitor of NADH, NHI-2 not only affects glycolysis but also influences mitochondrial metabolism and the NAD+/NADH balance [2].

Cell Viability Assay

Cell Line:	B78-D14 melanoma cells
Concentration:	0.1-100 μM
Incubation Time:	48 hours
Result:	The EC50 values of 32 μM.

Cell Proliferation Assay

Cell Line:	B78-D14 melanoma cells
Concentration:	12, 18, 26, 40, μM
Incubation Time:	72 hours
Result:	Exhibited no effect on B78 cell proliferation at lower concentrations. but decreased cell proliferation at concentrations beginning from 26 μM.

Apoptosis Analysis

Cell Line:	B78-D14 melanoma cells
Concentration:	40 μM
Incubation Time:	24 hours
Result:	Increased cell apoptosis when combined with either 30 or 60 mM Oxa

Cell Cycle Analysis

Cell Line:	B78-D14 melanoma cells
Concentration:	40 μM
Incubation Time:	24 hours
Result:	Led to an increased cell population in the S and G2 phases.

体内研究

The combination of NHI-2 (0.9 mg/kg; intratumoral injection; once daily for 15 days) with immune checkpoint inhibitors can inhibit tumor volume in the B78 melanoma tumor model in mice [2].

Animal Model:	C57BL/6 female mice (6-8 weeks) were intradermally implanted with B78 syngeneic melanoma cells
Dosage:	0.9 mg/kg
Administration:	Intra-tumoral (IT) via percutaneous needle puncture; once daily for 15 days
Result:	Suppressed tumor volume when combined with checkpoint inhibitors (ICIs).

分子式

C₁₇H₁₂F₃NO₃

分子量

335.2773296

CAS号

1269802-97-2

中文名称

NHI 2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 25 mg/mL (74.56 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9826 mL	14.9129 mL	29.8258 mL
5 mM	0.5965 mL	2.9826 mL	5.9652 mL
10 mM	0.2983 mL	1.4913 mL	2.9826 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： ≥98%

Data Sheet

产品使用指南

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