Gallocyanine - CAS:1562-85-2

您当前的位置：

Gallocyanine (Synonyms:Gallocyanine chloride ；花菁（没食子蓝）)

目录号 : KCM11687 CAS No. : 1562-85-2 纯度 : ≥95%

Data Sheet SDS 产品使用指南

Gallocyanine is a small molecule blue dye used to stain nuclei. It is known to inhibit Dickkopf-1 (Dkk1), an antagonist of the Wnt pathway. Gallocyanine can disrupt the interaction of Dkk1 with its receptor LRP5/6 (IC = 3 µM), thereby activating Wnt/β-catenin signaling. Modulation of the Dkk1-LRP5/6 interaction via gallocyanine has been shown to inhibit prostaglandin J-induced tau phosphorylation at serine in primary cortical neurons in vitro.

规格	价格	是否有货	数量
10 g		In-stock
25g		In-stock

加入购物车大包装询价

Other Forms of Rapamycin:

β-catenin/CBP-IN-1 In-stock
1H-Pyrazole-4-carboxamide, 5-methyl-1-phenyl-N-2-quinolinyl- In-stock
Gossypolone In-stock
PRI-724 In-stock
SRI 37892 In-stock

KKL Med 的所有产品和服务仅用于科学研究，不能被用于人体，兽医，我们也不向个人提供产品和服务。

生物活性

Gallocyanine （Gallocyanine chloride）is a synthetic blue dyestuff that can be used as a potential agent for the research of Alzheimer's disease and related neurodegenerative tauopathies. Gallocyanine （Gallocyanine chloride） inhibits DKK1/LRP6 interaction (IC50=6.38 μM), activates Wnt signaling pathway, and causes β-catenin accumulation. Gallocyanine （Gallocyanine chloride） exhibits anti-metastasis, anti-inflammatory and anti-fibrosis activities. Gallocyanine （Gallocyanine chloride） can be used as a fluorescent probe for detection of superoxide anion radicals.

体外研究

Gallocyanine chloride (0-20 μM, 48 h) promotes the proliferation of breast cancer cells MDA-MB-231 and 4T1, inhibits lipid peroxidation, and increases the levels of GSH and SLC7A11.

Cell Proliferation Assay

Cell Line:	MDA-MB-231 and 4T1
Concentration:	0-20 μM
Incubation Time:	48 hours
Result:	Increased the cell number.

体内研究

Gallocyanine （Gallocyanine chloride）(10 mg/kg, intravenous injection, once every 2 days, lasting for 3 weeks) can inhibit lung metastasis of breast cancer in the mouse MDA-MB-231 xenotransplantation model.
Gallocyanine （Gallocyanine chloride） (1 mg/kg, administered via tracheal instillation once a week for 4 weeks) can alleviate PM2.5 induced lung injury and fibrosis in a mouse model.

Animal Model:	Mouse MDA-MB-231 xenograft models
Dosage:	10 mg/kg
Administration:	iv, every 2 days for 3 weeks
Result:	Reduced the number and size of lung metastases, prolonged the mouse survival time.

Animal Model:	Mouse PM2.5-induced lung fibrosis models
Dosage:	1 mg/kg
Administration:	intratracheal instillation, once weekly for 4 weeks
Result:	Reduced levels of α-SMA and collagen in lung tissue, reduced levels of IL-1β and TNF-α in bronchoalveolar lavage fluid.

分子式

C₁₅H₁₃N₂O₅^.Cl

分子量

336.73

CAS号

1562-85-2

中文名称

花菁

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 11.67 mg/mL (34.66 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9697 mL	14.8487 mL	29.6974 mL
5 mM	0.5939 mL	2.9697 mL	5.9395 mL
10 mM	0.2970 mL	1.4849 mL	2.9697 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 0.53 mg/mL (1.57 mM); Clear solution

此方案可获得 ≥ 0.53 mg/mL (1.57 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 5.30 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂：10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 0.53 mg/mL (1.57 mM); Clear solution

此方案可获得 ≥ 0.53 mg/mL (1.57 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 5.30 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。