17α,20β-Dihydroxy-4-pregnen-3-one - CAS:1662-06-2

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17α,20β-Dihydroxy-4-pregnen-3-one (Synonyms:4-孕烷-17α,20β-二醇-3-酮；17,20β-P; 17,20β-DHP)

目录号 : KCM11892 CAS No. : 1662-06-2 纯度 : ≥95%

Data Sheet 产品使用指南

17α,20β-Dihydroxy-4-pregnen-3-one (17α,20β-DHP) is an endogenous, maturation-inducing steroid that stimulates oocyte maturation in females of several teleost species. For example, 1 µg/ml of 17α,20β-DHP applied to Persian sturgeon oocytes has been shown to effectively induce germinal vesicle breakdown, an essential step during oocyte maturation. Gonadotropin-releasing hormone and the gonadotropins, follicle-stimulating hormone and luteinizing hormone, have been shown to stimulate the production of 17α,20β-DHP either in vivo or in vitro. 17α,20β-DHP has also been reported to influence spermiation by stimulating milt production when administered at 5 mg/kg to various male teleosts.

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

17α,20β-Dihydroxy-4-pregnen-3-one acts as the maturation-inducing hormone (MIH) in salmonid fish and rainbow trout. 17α,20β-Dihydroxy-4-pregnen-3-one binds to oocyte-specific plasma membrane receptors and pertussis toxin-sensitive inhibitory G proteins in fish, inhibits Adenylate Cyclase and reduces intracellular cAMP via the membrane receptor-G protein coupling pathway, thereby initiating downstream signal transduction. 17α,20β-Dihydroxy-4-pregnen-3-one induces de novo synthesis of cyclin B, mediates the translation of cyclin B mRNA and the phosphorylation of cdc2, and promotes the production of maturation-promoting factor (MPF). 17α,20β-Dihydroxy-4-pregnen-3-one drives meiotic maturation of fish oocytes. 17α,20β-Dihydroxy-4-pregnen-3-one is applicable for development-related research.

体外研究

17α,20β-Dihydroxy-4-pregnen-3-one There are two types of high affinity (K_d=18 nM) and low affinity (K_d=0.3 μM) receptors that can bind to the membrane of rainbow trout oocytes in the late stage of yolk formation, and their signaling pathways involve pertussis toxin sensitive inhibitory G protein (G_i ) .
17 α, 20 β - Dihydroxy-4-pregnen-3-one is the most potent steroid in inducing oocyte maturation in amago salmon, and its endogenous levels sharply increase during oocyte maturation and ovulation; It can inhibit the activity of adenylate cyclase in the membrane of rainbow trout oocytes, which is partially mediated by the alpha subunit of inhibitory G protein (G_i ) .
17α,20β-Dihydroxy-4-pregnen-3-one (100 nM-1 μM) Selective reduction of ADP ribosylation of the 40 kDa G_i α subunit in the late stage of rainbow trout yolk formation catalyzed by pertussis toxin indicates its ability to activate inhibitory G protein.
17 α, 20 β - Dihydroxy-4-pregnen-3-one can induce complete maturation of immature goldfish oocytes through a membrane mediated pathway, initiating de novo synthesis of cyclin B by prolonging the cytoplasmic 3'poly (A) of pre-existing cyclin B mRNA.
17 α, 20 β - Dihydroxy-4-pregnen-3-one can induce the activation of maturation promoting factor (MPF) in goldfish oocytes by triggering de novo synthesis of cyclin B; The newly synthesized cyclin B binds to the inactive 35 kDa cdc2 kinase and promotes the phosphorylation of Thr¹⁶¹, thereby generating an active cdc2 kinase.

分子式

C₂₁H₃₂O₃

分子量

332.48

CAS号

1662-06-2

中文名称

4-孕酮-17α,20β-二醇-3-酮;二(硫代过氧基二甲酰)四丁二胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (300.77 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0077 mL	15.0385 mL	30.0770 mL
5 mM	0.6015 mL	3.0077 mL	6.0154 mL
10 mM	0.3008 mL	1.5038 mL	3.0077 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.52 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。