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L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation.

L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) magnesium hydrate significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect.
L-Ascorbic acid 2-phosphate (50 μM-250 μM) magnesium hydrate is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex.

2-8°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

H2O : 18.42 mg/mL (ultrasonic and warming and heat to 60°C)