Chlorothalonil
(Synonyms:百菌清)
目录号 : KCM12239
CAS No. : 1897-45-6
纯度 : ≥98%
Chlorothalonil is a broad-spectrum organochlorine fungicide that forms adducts with glutathione (GST; ) and cysteine residues on enzymes leading to GST depletion and enzyme deactivation, respectively. In vitro, it inhibits the growth of C. albicans and C. orbiculare fungi, S. aureus and B. cereus Gram-positive bacteria, and E. coli and P. aeruginosa Gram-negative bacteria (MICs = 0.7, 5, 1.3, 0.7, 0.5, and 1.7 µg/ml, respectively). In vivo, chlorothalonil (100 µg/ml) completely inhibits the growth of P. infestans, the tomato late blight pathogen, on tomato plants. It is toxic to aquatic organisms, including species of fish, crustaceans, molluscs, and algae with tenth percentile of toxicity values of 25.23, 40.59, 0.69, and 3.94 µg/L, respectively, as well as to other aquatic invertebrates. Chlorothalonil is carcinogenic in animal models and induces neoplasms in the forestomach and kidneys of rats when administered at a dose of 3.8 mg/kg per day, but it is not genotoxic. Formulations containing chlorothalonil have been used as fungicides in agriculture.
Other Forms of Rapamycin:
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| 生物活性 |
Chlorothalonil is a broad-spectrum foliar fungicide with oral activity. Chlorothalonil can be used to combat fungal diseases in vegetable and crop leaves. Chlorothalonil can alter the microbial community in the soil. Chlorothalonil inhibits spermatogenesis. Chlorothalonil can cause intestinal epithelial barrier dysfunction and fetal toxicity.
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| 体外研究 |
Chlorothalonil (10mg/kg soil) can inhibit the activity of phosphatase and dehydrogenase in soil, and reduce the number of microorganisms in soil .
Chlorothalonil (0.6-4.8 µg/mL; Induced intestinal epithelial barrier (IEB) dysfunction by activating the mitogen activated protein kinase (MAPK) pathway in Caco-2 cells over a period of 4 days.
Chlorothalonil (0.1-10 μM; 4 hours and 24 hours) reduced pig sperm motility and increased sperm apoptosis in a concentration - and time-dependent manner.
RT-PCR
| Cell Line: |
Caco-2 (Human colorectal adenocarcinoma cell line) |
| Concentration: |
0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL, 4.8 µg/mL |
| Incubation Time: |
1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 8 days |
| Result: |
Down-regulated the mRNA levels of TJ genes (ZO-1, OCLN, and CLDN1), polar marker genes (SI), and anti-apoptotic genes (BCL-2), and up-regulated the mRNA levels of apoptosis-related genes (BAD, BAX, CASP3, and CASP8). |
Western Blot Analysis
| Cell Line: |
Caco-2 (Human colorectal adenocarcinoma cell line) |
| Concentration: |
0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL |
| Incubation Time: |
4 days |
| Result: |
Decreased levels of the TJ protein network (ZO-1 and CLDN1).
Increased the expression levels of P-ERK1/2, PJNK and P-p38 and reduced levels of ERK1/2, JNK and p38.( All are mitogen-activated protein kinase (MAPK) signaling pathway-related proteins) |
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| 体内研究 |
Chlorothalonil (400-600 mg/kg; oral; Once a day for 18 consecutive days can cause toxicity and fetal toxicity in pregnant ICR mice.
Chlorothalonil (0.1-10 mg/kg; oral; Once a day for 5 consecutive weeks will reduce sperm motility and inhibit spermatogenesis in ICR mice.
| Animal Model: |
ICR mouse model of pregnancy |
| Dosage: |
100 mg/kg, 400 mg/ kg, 600 mg/kg |
| Administration: |
Oral gavage (p.o.); Once daily for 18 days |
| Result: |
Reduced weight gain in pregnant mice at doses of 400 mg/kg and 600 mg/kg throughout pregnancy by 36% and 48%, respectively.
Significantly increased embryo mortality at doses of 400 mg/kg and 600 mg/kg.
Significantly reduced number of live births (28%).
Reduced mean fetal weight (22% to 39%) at doses of 400 and 600 mg/kg. |
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| Animal Model: |
ICR male mice model |
| Dosage: |
0.1mg/kg, 1 mg/ kg, 10 mg/kg |
| Administration: |
Oral gavage (p.o.); Once daily for 5 weeks |
| Result: |
Decreased sperm motility in ICR mice.
At doses of 1 mg/kg and 10 mg/kg, decreased the protein factors A-myb, GDNF and DDX4, and finally interrupted the spermatogenesis process.
Decreased the sperm marker Acrosin and PNA in a dose-dependent manner at doses of 1 mg/kg and 10 mg/kg.
At doses of 1 mg/kg and 10 mg/kg, Significantly reduced the number of estrogen receptor Alpha (ERα) positive stromal cells in mouse testis.
Decreased AKT levels in the testis, but did not change p-AKT levels.
Disrupted the methylation of histones and DNA. |
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| 分子式 |
C8Cl4N2
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| 分子量 |
265.91
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| CAS号 |
1897-45-6
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| 中文名称 |
百菌清
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
| Powder |
-20°C |
3 years |
| |
4°C |
2 years |
| In solvent |
-80°C |
6 months |
| |
-20°C |
1 month |
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| 溶解性数据 |
In Vitro:
DMSO : 33.33 mg/mL (125.34 mM; Need ultrasonic)
配制储备液
| 浓度 溶剂体积 质量 |
1 mg |
5 mg |
10 mg |
| 1 mM |
3.7607 mL |
18.8034 mL |
37.6067 mL |
| 5 mM |
0.7521 mL |
3.7607 mL |
7.5213 mL |
| 10 mM |
0.3761 mL |
1.8803 mL |
3.7607 mL |
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶
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1.
请依序添加每种溶剂：10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline
Solubility: ≥ 2.5 mg/mL (9.40 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.40 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
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2.
请依序添加每种溶剂：10% DMSO → 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.40 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.40 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
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Data Sheet
