DMU-212 - CAS:134029-62-2

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DMU-212

目录号 : KM6270 CAS No. : 134029-62-2 纯度 : 98%

Data Sheet 产品使用指南

DMU-212 是一种白藜芦醇甲基化衍生物，具有抗分裂、抗增殖、抗氧化和促进细胞凋亡的活性。DMU-212 通过诱导凋亡 (apoptosis) 和激活 ERK1/2 蛋白导致有丝分裂停止。DMU-212 具有口服活性。

规格	价格	是否有货
10mg		In-stock
25mg		In-stock
50mg		In-stock
100mg	询价	In-stock
200mg	询价	In-stock

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生物活性

DMU-212 is a methylated derivative of Resveratrol , with antimitotic, anti-proliferative, antioxidant and apoptosis promoting activities. DMU-212 induces mitotic arrest via induction of apoptosis and activation of ERK1/2 protein. DMU-212 has orally active.

体外研究

DMU-212 (0.3125-40 μM) inhibits growth of A375, MeWo, Bro and M5 human melanoma cells.
DMU-212 (30-50 μM; 24 hours) induces upregulation of cell cycle inhibitors, apoptosis and ERK activation in A375 cells.

Cell Proliferation Assay

Cell Line:	A375 cells, MeWo cells, M5 cells, Bro cells
Concentration:	0.3125 μM, 0,625 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM
Incubation Time:	96 hours
Result:	Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC₅₀=0.5 μM for A375 and Bro and IC₅₀= 1.25 μM for MeWo and M5 cells).

Cell Cycle Analysis

Cell Line:	A375 cells
Concentration:	20 μM, 30 μM, 50 μM
Incubation Time:	24 hours
Result:	Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2.

Western Blot Analysis

Cell Line:	A375 cells
Concentration:	20 μM, 30 μM, 50 μM
Incubation Time:	24 hours
Result:	Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2.

Apoptosis Analysis

Cell Line:	A375 cells
Concentration:	10 μM, 20 μM
Incubation Time:	24 hours, 36 hours
Result:	Induced apoptosis.

体内研究

DMU-212 (50 mg/kg; i.g.; three times a week; for 14 days) inhibits tumor growth in xenograft model of human ovarian cancer.

Animal Model:	6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts
Dosage:	50 mg/kg
Administration:	Oral gavage, three times a week, for 14 days
Result:	Lowered tumor burden.

分子式

C₁₈H₂₀O₄

分子量

300.35

CAS号

134029-62-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 20 mg/mL (66.59 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.3294 mL	16.6472 mL	33.2945 mL
5 mM	0.6659 mL	3.3294 mL	6.6589 mL
10 mM	0.3329 mL	1.6647 mL	3.3294 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2 mg/mL (6.66 mM); Clear solution

此方案可获得 ≥ 2 mg/mL (6.66 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。