Daunorubicin - CAS:20830-81-3

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Daunorubicin (Synonyms:柔红霉素; Daunomycin; RP 13057; Rubidomycin)

目录号 : KG10889 CAS No. : 20830-81-3 纯度 : 98%

Data Sheet SDS 产品使用指南

Daunorubicin is an inhibitor of DNA topoisomerase II . Daunorubicin is an anthracycline antibiotic. It is also used as an effective chemotherapeutic agent against tumors especially acute myeloid leukaemia and acute lymphocytic leukaemia. Daunorubicin can affect the metabolism and synthesis of DNA and RNA. In the in vitro assay, daunorubicin inhibits the incorporation of thymidine and uridine into L1210 cells. It also inhibits the incorporation of labeled precursors into the isolated DNA and RNA from incubated cells. When treated with leukemic cells isolated from acute lymphocytic leukemia patients, daunorubicin significantly inhibits the biosynthesis of the DNA and RNA macromolecules .

规格	价格	是否有货	数量
10mg		In-stock	立即试用
50mg		In-stock

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生物活性

Daunorubicin is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin inhibits DNA and RNA synthesis. Daunorubicin is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin is also an anthracycline antibiotic. Daunorubicin can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, Wilms' tumor.

体外研究

Daunorubicin (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells.

Daunorubicin (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells.

Daunorubicin (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells.

Daunorubicin (0.4 μM, 120 min) induces ROS generation in L3.6 cells.

Daunorubicin (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line).

Cell Viability Assay

Cell Line:	Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line)
Concentration:	7 nM-1.9 μM
Incubation Time:	72 hours
Result:	Inhibited cell viability with IC50 values of 40 nM (Molt-4) and 400 nM (L3.6).

Apoptosis Analysis

Cell Line:	L3.6 cells
Concentration:	0.4 μM
Incubation Time:	24, 48 hours
Result:	Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h.

Western Blot Analysis

Cell Line:	K562 cells
Concentration:	2 μM
Incubation Time:	24 hours
Result:	Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.

体内研究

Daunorubicin (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats.

Daunorubicin (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice.

Animal Model:	Male Sprague-Dawley rats
Dosage:	3 mg/kg
Administration:	Intravenous injection, three times at 48 h intervals.
Result:	Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity. Increased urinary protein excretion, serum creatinine, and BUN level.

分子式

C₂₇H₂₉NO₁₀

分子量

527.52

CAS号

20830-81-3

中文名称

道诺霉素;红保霉素;红比霉素;红卫霉素;柔红霉素;柔毛霉素;正定霉素;柔红霉素,道诺霉素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO: 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8957 mL	9.4783 mL	18.9566 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： 98%

Data Sheet

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