Strophanthidin - CAS:66-28-4

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Strophanthidin (Synonyms:葡萄球菌素)

目录号 : KM26589 CAS No. : 66-28-4 纯度 : 92%

Data Sheet SDS 产品使用指南

Strophanthidin is a naturally available cardiac glycoside. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 µmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal. Strophanthidin increases both diastolic and systolic intracellular Ca2+ concentration.

规格	是否有货	数量
5mg	In-stock	立即试用
10mg	In-stock
25mg	In-stock

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生物活性

体外研究

Strophanthidin (0-10 μM; 24 hours; MCF-7, A549, and HepG2 cells can effectively inhibit cancer cell growth and are non-toxic to normal cells.
Strophanthidin (0.5 - 500 μM； PBMC did not show significant cytotoxicity in PBMC. Strophanthidin (2 μM; MCF-7 cells) can arrest the cell cycle at the G2/M phase.
Strophanthidin (MCF-7, A549, and HepG2 cells) can effectively inhibit cancer cell growth and is non-toxic to normal cells. Compared with the untreated control group, Strophanthidin (MCF-7, A549, and HepG2 cells) can inhibit the expression of checkpoint and cell cycle protein dependent kinases in three types of cancer cells.
Strophanthidin can regulate the localization of protein from the nucleus to the cell membrane and cytoplasm. Strophanthidin is a monosaccharide cardiac glycoside that contains a glycosidic ligand moiety and no sugar units. Strophanthidin induces cell apoptosis by inhibiting multiple biochemical signaling pathways and halting the cell cycle in the G2/M phase through p53 dependent and non p53 dependent mechanisms.

Cell Viability Assay

Cell Line:	MCF-7, A549, and HepG2 cells
Concentration:	0~10 μM
Incubation Time:	24 hours
Result:	Inhibited the proliferation in three different cancer cells.

分子式

C₂₃H₃₂O₆

分子量

404.5

CAS号

66-28-4

中文名称

毒毛旋花苷配基

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

Ethanol : 25 mg/mL (61.80 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4722 mL	12.3609 mL	24.7219 mL
5 mM	0.4944 mL	2.4722 mL	4.9444 mL
10 mM	0.2472 mL	1.2361 mL	2.4722 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% EtOH → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.18 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% EtOH → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.18 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% EtOH → 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.18 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中，混合均匀。