Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) (Synonyms:5(6)-ROX, SE, 混合物;5(6)-羧基SNARF-1, 乙酰甲酯, 乙酸酯(5MM 无水DMSO溶液))
目录号 : KM31382 CAS No. : 126208-13-7 纯度 : 98%
Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) is a fluorescent probe that is sensitive to pH. Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture) can be used for measurement of intracellular pH.
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50mg 询价 In-stock
100mg 询价 In-stock
250mg 询价 In-stock

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生物活性

The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH.

体外研究

Incubating cells in 1~10 μM SNARF AM acetate for 30 minutes at the optimum temperature for the specific cell type of interest is recommend. Then, cells should be washed before pH measurements.

分子式
C32H25NO9
分子量
567.54
CAS号
126208-13-7
中文名称
5(6)-羧基SNARF-1,乙酰甲酯, 乙酸酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2