Spliceostatin A - CAS:391611-36-2

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Spliceostatin A

目录号 : KM32509 CAS No. : 391611-36-2 纯度 : 96%

Data Sheet 产品使用指南

Spliceostatin A，是 FR901464 的甲基化衍生物，是一种有效的抗肿瘤活性分子。Spliceostatin A 通过结合 SF3B1 抑制剪接并促进前体 mRNA 的积累。SF3B1 是剪接体中 U2 小核核糖核蛋白的一个亚复合体。Spliceostatin A 诱导慢性淋巴细胞白血病 (CLL) 细胞凋亡 (Apoptosis)。

规格	价格	是否有货	数量
100μg		In-stock

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Other Forms of Rapamycin:

(R)-9b In-stock
Spliceostatin A In-stock
JBI-589 In-stock
PF-04523655 In-stock
EP300/CBP-IN-2 In-stock

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生物活性

Spliceostatin A, the FR901464 methylated derivative, is a potent anti-tumor agent. Spliceostatin A inhibits splicing and promotes pre-mRNA accumulation by binding SF3B1. SF3B1 is a subcomplex of U2 small nuclear ribonucleoprotein in the spliceosome. Spliceostatin A induces Apoptosis in chronic lymphocytic leukemia (CLL) cells.

体外研究

Spliceostatin A (2.5-20 nM; 0-24 h) induces Caspase dependent apoptosis in CLL cells in a dose - and time-dependent manner [1].
Spliceostatin A (10 nM, 20 nM; 24 h) has a synergistic effect with ABT-199 or ABT-263, increasing apoptosis of CLL cells after treatment with IL4/CD40L (10 ng/mL, 300 ng/mL; 6 h) and reducing related protein phosphorylation levels [1].
Spliceostatin A inhibits the activity of normal B (CD19+) and T (CD3+) lymphocytes, with IC50 values of 12.1 nM and 61.7 nM, respectively [1].

Apoptosis Analysis

Cell Line:	CLL cells
Concentration:	0, 2.5 nM, 5 nM, 10 nM, and 20 nM
Incubation Time:	0, 6 h, 12 h, and 24 h
Result:	Increased cell apoptosis

Western Blot Analysis

Cell Line:	CLL cells
Concentration:	10 nM, and 20 nM
Incubation Time:	24 h; treated after 10 ng/mL IL4 and/or 300 ng/mL CD40L for 6 h
Result:	Decreased the level of PARP, phosphorylated STAT6 (pSTAT6), phosphorylated IκBα (pIκB α), Mcl-1L, Bcl-xL , Bcl-2 and Hsc70.

分子式

C₂₈H₄₃NO₈

分子量

521.643

CAS号

391611-36-2

运输条件

Shipping with dry ice.

储存方式

-80°C, protect from light

溶解性数据

In Vitro:

DMSO: 50 mg/mL (95.85 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9170 mL	9.5852 mL	19.1703 mL
5 mM	0.3834 mL	1.9170 mL	3.8341 mL
10 mM	0.1917 mL	0.9585 mL	1.9170 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months (protect from light)。-80°C 储存时，请在 6 个月内使用。

纯度： 96%

Data Sheet

产品使用指南

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Other Forms of Rapamycin:

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