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Sodium sulfite (Synonyms:亚硫酸钠)
目录号 : KM32565 CAS No. : 7757-83-7 纯度 : 98%
Data Sheet 产品使用指南

Sodium sulfite 是一种无机盐，可作为抗氧化剂和防腐剂。Sodium sulfite 还用于磺化和磺甲基化反应。Sodium sulfite 也可用作漂白剂、除硫剂和脱氯剂。Sodium sulfite 可抑制肝细胞增殖，促进肝细胞凋亡和坏死，破坏线粒体完整性。Sodium sulfite 可诱导超氧阴离子生成，使中性粒细胞为增强超氧阴离子生成做好准备，并诱导中性粒细胞基因表达。Sodium sulfite 可用于肺部炎症和胃组织损伤相关的研究。

规格 价格 是否有货 数量
100g
In-stock
500g
In-stock
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生物活性

Sodium sulfite is an inorganic salt used as an antioxidant and preservative. Sodium sulfite is also used in sulfonation and sulfomethylation reactions. Sodium sulfite can also be used as a bleaching agent, desulfurizer, and dechlorinator. Sodium sulfite inhibits hepatocyte proliferation, promotes hepatocyte apoptosis and necrosis, and impairs mitochondrial integrity. Sodium sulfite induces superoxide anion production, primes neutrophils for enhanced superoxide anion generation, and induces neutrophil gene expression. Sodium sulfite can be used in studies related to pulmonary inflammation and gastric tissue injury.
体外研究

Sodium sulfate (10-5-10-2 M; 24-72 h) can alter the morphology of human liver cell L02 in a time - and concentration dependent manner, and has significant toxic effects at concentrations of 10-3 M and 10-2 M [1].
Sodium sulfide (10-5-10-2 M; 72 h) can concentration dependently inhibit the viability of human liver cell L02, with an IC50 of 4.68 × 10-4 M after 72 h of incubation [1].
Sodium sulfide (10-5-10-2 M; 24-72 h) can inhibit the proliferation of human liver cell L02 in a time - and concentration dependent manner. When the concentration reaches 5 × 10-4 M or above, it can produce significant inhibitory effects [1].
Sodium sulfide (10-5-10-2 M; 72 h) induces apoptosis in human liver cell L02 in a concentration dependent manner, with significant apoptosis occurring at 10-4 M and severe cell death occurring within 24 h under 10-2 M conditions [1].
Sodium sulfate (4.68 × 10-4 M; 72 h) can alter the expression of 97 genes in human liver cell L02, with 13 genes significantly upregulated and 14 genes significantly downregulated; This substance can activate pathways that inhibit cell proliferation, induce cell apoptosis, and cause mitochondrial damage through the generation of reactive oxygen species.
Sodium sulfide (0.1-10 mM; 5-30 min) can induce the production of reactive oxygen species in freshly isolated human neutrophils in a concentration dependent manner within 5-30 min; After 30 minutes of pre incubation (0.1-10 mM; 30 min pre incubation followed by 5 min fMLP stimulation), these neutrophils were concentration dependent activated, enhancing fMLP induced reactive oxygen species production [2].
Sodium sulfide (0.1-10 mM; 4 h) can induce RNA synthesis in freshly isolated human neutrophils in a concentration dependent manner, but does not cause morphological changes in neutrophils [2].

Sodium sulfate (0.1-10 mM; 20 h) does not regulate the apoptosis rate of freshly isolated human neutrophils after 20 h incubation, nor can it reverse the delayed apoptosis effect of GM-CSF on these cells [2].
Sodium sulfate (0.001-10 mM; 5-24 h) can induce dose-dependent necrosis of RGM1 cells through oxidative stress, with an IC50 of 0.17 mM, and this cytotoxicity can be attenuated by the free radical scavenger Trolox [3].
Sodium sulfide (0-5 mM; 20 h) can induce necrotic cell death in RGM1 cells, and this process is independent of the apoptotic signaling pathway; This can be confirmed by unchanged caspase/PARP cleavage, caspase activity, DNA fragmentation, and dose-dependent LDH release [3].
Sodium sulfate (0-10 mM; 0-120 min, 24 h) can induce oxidative modification, aggregation, and inactivation of purified lysozyme through metal ion catalyzed free radical formation, without relying on the clearance of superoxide anions or hydrogen peroxide [3].

Cell Viability Assay

Cell Line: human hepatocytes L02
Concentration: 10-5 M, 10-4 M, 2.5×10-4 M, 5×10-4 M, 5×10-3 M, 10-2 M
Incubation Time: 72 hours
Result:
Gradually decreased L02 cell viability with increasing sodium sulfite concentration.
Reached an IC50 of 4.68×10-4 M for L02 cells at 72 h.

Cell Proliferation Assay

Cell Line: human hepatocytes L02
Concentration: 10-5 M, 10-4 M, 5×10-4 M, 5×10-3 M, 10-2 M
Incubation Time: 24 h, 48 h, 72 h
Result: Significantly inhibited L02 cell proliferation at concentrations ≥5×10-4 M, with proliferation rate decreasing as incubation time increased.
Had no obvious effect on proliferation activity at low concentrations (10-5 M, 10-4 M).

Apoptosis Analysis

Cell Line: human hepatocytes L02
Concentration: 10-5 M, 10-4 M, 10-3 M, 10-2 M
Incubation Time: 72 hours
Result: Caused cell color changes from green to orange/red (indicating apoptosis) at 10-4 M with increased incubation time.
Induced significant apoptosis as early as 24 h at 10-3 M, indicated by dominant orange and red staining.
Led to majority of cells being dead by 24 h at 10-2 M.

Real Time qPCR

Cell Line: human hepatocytes L02
Concentration: 4.68×10-4 M (IC50)
Incubation Time: 72 hours
Result: Identified 97 significantly regulated differentially expressed genes between sodium sulfite-treated and control cells via transcriptomic analysis.
Confirmed the expression changes of 13 significantly upregulated genes (including SOCS3, FOSL1, PLK3, IL1B) and 14 significantly downregulated genes (including CPS1, FBN1, FKBP3) via qPCR.
Linked these genes to pathways including TNF signaling, IL-17 signaling, apoptosis, metabolism, and inflammatory responses.

Apoptosis Analysis

Cell Line: rat gastric mucosal epithelial cells (RGM1)
Concentration: 0-5 mM (20 h incubation); 0-5 mM (1 h pre-incubation followed by 20 h in fresh medium)
Incubation Time: 20 h; 1 h (pre-incubation) + 20 h (fresh medium)
Result: Did not alter levels of cleaved caspase-9, cleaved caspase-3, or cleaved PARP, nor change caspase-9 or caspase-3 activity.
Showed no apoptotic DNA fragmentation, but dose-dependently increased LDH release into the culture medium.
分子式
Na2SO3
分子量
126.04
CAS号
7757-83-7
中文名称
亚硫酸钠
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

2-8°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

H2O : ≥ 200 mg/mL (1586.80 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
                                           
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
                                                                                                                    
1 mM 7.9340 mL 39.6699 mL 79.3399 mL
5 mM 1.5868 mL 7.9340 mL 15.8680 mL
10 mM 0.7934 mL 3.9670 mL 7.9340 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
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