3,3′-Dioctadecyloxacarbocyanine perchlorate is a green fluorescent lipophilic tracer, which is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.
体外研究
A fluorescent dialkyl carbocyanine dye (DiOC18(3)) is used to label muscle membranes, and the membranes accessible to the dye were observed by confocal laser scanning microscopy. A loss of cell membrane integrity results from contractile activity, enabling DiOC18(3) staining of internal membranes. The resulting staining pattern is striking and fibers with damaged cell membranes are easily distinguished from uninjured ones. 3,3′-Dioctadecyloxacarbocyanine perchlorate is a cationic oxacarbocyanine dye. The absorption and fluorescence spectra of 3,3'-dioctadecyloxacarbocyanine, a cationic oxacarbocyanine dye have been studied in aqueous and nonaqueous media containing egg phosphatidylcholine (PC) as well as in different solvents of diverse nature. The results show the evidence of complex formation of the dye in the ground and in the excited states with PC. The excited state interaction of the dye with PC suggests the electron transfer from PC to dye and this is supported by photovoltage generation in a photoelectrochemical cell consisting of dye and PC in aqueous medium.
分子式
C53H85ClN2O6
分子量
881.70
CAS号
34215-57-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
2-8°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:
DMF : 10 mg/mL (11.34 mM; Need ultrasonic)
DMSO : 5 mg/mL (5.67 mM; ultrasonic and warming and heat to 60°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.1342 mL
5.6709 mL
11.3417 mL
5 mM
0.2268 mL
1.1342 mL
2.2683 mL
10 mM
0.1134 mL
0.5671 mL
1.1342 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。