DiI (Synonyms:DiIC18(3))
目录号 : KM14226 CAS No. : 41085-99-8 纯度 : 98%

DiI (DiIC18(3)) 是一种亲脂性膜染料。DiI 在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiI 被激发后可以发出橙红色的荧光,最大激发波长为 549 nm,最大发射波长为 565 nm。通常用作神经元和其它细胞的长期示踪剂。

规格 价格 是否有货 数量
10mg
In-stock
25mg
In-stock
50mg
In-stock
100mg 询价 In-stock
200mg 询价 In-stock

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生物活性

DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.

体外研究

Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.

体内研究

DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo

分子式
C59H97ClN2O4
分子量
933.87
CAS号
41085-99-8
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 30 mg/mL (32.12 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0708 mL 5.3541 mL 10.7081 mL
5 mM 0.2142 mL 1.0708 mL 2.1416 mL
10 mM 0.1071 mL 0.5354 mL 1.0708 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

科研文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2