Stauprimide - CAS:154589-96-5

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Stauprimide (Synonyms:顺式1-苄基-4-甲基-3-甲氨基-哌啶盐酸盐;抑胃酶氨酰u丙基亚胺)

目录号 : KCM11651 CAS No. : 154589-96-5 纯度 : ≥98%

Data Sheet SDS 产品使用指南

Stauprimide is a small molecule that increases the efficiency of mouse and human embryonic stem cell differentiation in conjunction with lineage specification cues (EC = 0.3 μM). It specifically inhibits the nuclear localization of NME2, which results in the suppression of c-Myc, a key regulator of pluripotency.

规格	价格	是否有货	数量
500μg		In-stock
1mg		In-stock
5mg		In-stock

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Other Forms of Rapamycin:

CBP/p300-IN-2 In-stock
Moracin T In-stock
Stauprimide In-stock

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生物活性

Stauprimide is a staurosporine analog that promotes embryonic stem cell (ESC) differentiation. Stauprimide is a non-broad spectrum inhibitor that binds to the MYC transcription factor NME2 and blocks its nuclear localization in ESCs, which results in down-regulation of MYC transcription.

体外研究

Staupriide (10 μM; 6 Hours) inhibits MYC transcription in most cell lines, with EC50 ranging from 30 nM to 8 μM, and reduces MYC levels by 15% to over 90%.
Staupriide (2-8 μM; 24-72 hours) downregulates MYC leading to in vitro cell proliferation inhibition, with an IC₅₀ of 780 nM in RXF 393 cells.
Staupriide (5 μM; 3 hours) inhibits MYC transcription by reducing NME2 nuclear translocation.

Cell Proliferation Assay

Cell Line:	Renal cancer cell line RXF 393 cells
Concentration:	2 μM, 4 μM, 8 μM
Incubation Time:	24 hours, 48 hours, 72 hours
Result:	Inhibited cell proliferation at concentrations of 2-8 μM.

Western Blot Analysis

Cell Line:	Renal cancer cell line RXF 393 cells and CAKI-1 cells
Concentration:	5 μM
Incubation Time:	3 hours
Result:	Decreased nuclear localized NME2.

RT-PCR

Cell Line:	CA46 cells; Ramos cells; RXF393 cells; TK10 cells; KG1A cells; CAKI-1 cells
Concentration:	4μM, 6μM, 8μM, 10μM
Incubation Time:	6 hours
Result:	Suppressed MYC transcription in KG1A cells with an EC₅₀ of 400±50 nM and a suppression of 90%; CA46 cells were resistant to stauprimide.

体内研究

Staupriide (oral administration; 50 mg/kg； Once a day; 30 days, 55 days) to block tumor growth, reduce MYC protein levels in RXF 393 or CAKI-1 cell xenograft mice, and inhibit MYC transcription in RXF 393 tumors.

Animal Model:	Xenograft tumor models with RXF 393 and CAKI-1 cells in NOD/SCID mice
Dosage:	50 mg/kg
Administration:	Oral administration; 50 mg/kg; once daily; 30 days, 55 days
Result:	Blocked tumor growth in mice injected with either RXF 393 or CAKI-1 cells during the dosing periods.

分子式

C₃₅H₂₈N₄O₅

分子量

584.62

CAS号

154589-96-5

中文名称

抑胃酶氨酰u丙基亚胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据

In Vitro:

DMSO: 10 mg/mL (17.11 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7105 mL	8.5526 mL	17.1051 mL
5 mM	0.3421 mL	1.7105 mL	3.4210 mL
10 mM	0.1711 mL	0.8553 mL	1.7105 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： ≥98%