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Eicosapentaenoyl serotonin (EPA-5-HT) is an endogenous fatty acid-serotonin conjugate lipid mediator. Eicosapentaenoyl serotonin acts as an inhibitor of fatty acid amide hydrolase (FAAH). Eicosapentaenoyl serotonin suppresses IL-17 release in Concanavalin A stimulated human peripheral blood mononuclear cells. Eicosapentaenoyl serotonin is regulated by polyunsaturated fatty acids and modulates intestinal immunity and Th17 signaling. Eicosapentaenoyl serotonin can be used for the study of inflammatory bowel disease-related mechanisms.

Eicosapentaenoyl serotonin (EPA-5-HT) (0-555 μM) can inhibit FAAH activity in a concentration dependent manner in vitro, and its efficacy is similar to that of eicosapentaenoic acid.
Eicosapentaenoyl serotonin (5 μM; 30 min pre-incubation; 24 h incubation with ConA)  It can significantly inhibit the production of IL-17 by human peripheral blood mononuclear cells induced by Concanavalin A , but has no effect on the production of IL-6.

Eicosapentaenoyl serotonin (fish oil accounts for 1-3%; Dietary administration; Free eating; At 6 weeks, fish oil can induce endogenous generation in a dose-dependent manner in the jejunum of healthy mice, with statistically significant increases observed at dietary fish oil levels of 1% and 3%.

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