Thiazolyl Blue - CAS:298-93-1

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Thiazolyl Blue (Synonyms:MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

目录号 : KM9350 CAS No. : 298-93-1 纯度 : 98%

Data Sheet SDS 产品使用指南

Thiazolyl Blue (MTT) 是一种广泛用于测量细胞增殖的比色剂。Thiazolyl Blue (MTT) 在活细胞中从黄色还原为紫色甲臜。

规格	价格	是否有货
500mg		In-stock
1g		In-stock
5g		In-stock
10g	询价	In-stock
50g	询价	In-stock

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Other Forms of Rapamycin:

Thiazolyl Blue In-stock

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生物活性

Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

体外研究

Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis.

分子式

C₁₈H₁₆BrN₅S

分子量

414.32

CAS号

298-93-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 25 mg/mL (60.34 mM; ultrasonic and warming and heat to 60°C)

H₂O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4136 mL	12.0680 mL	24.1359 mL
5 mM	0.4827 mL	2.4136 mL	4.8272 mL
10 mM	0.2414 mL	1.2068 mL	2.4136 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

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